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The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis <t>of</t> <t>ZO-1</t> (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
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Image Search Results


The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Journal: Natural Products and Bioprospecting

Article Title: Combined treatment with naringin and osthole ameliorates colitis through microbiota–amino acid metabolism and the JNK pathway

doi: 10.1007/s13659-025-00582-z

Figure Lengend Snippet: The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: ZO-1 , sc-33725 , 1: 200 (IF) , Mouse , Santa Cruz Biotechnology.

Techniques: Immunofluorescence, Staining, Activity Assay, Western Blot

HYP ameliorates intestinal inflammation and restores barrier integrity in DSS-induced colitis mice. mRNA expression levels of (A) IL-6, (B) IL-1β, and (C) TNF-α in colon tissues. Levels of (E) IL-6, (F) IL-1β, and (G) TNF-α in serum. Quantitative analysis of the positive area percentage for (D) Mucin-2 and (H) ZO-1. Representative images of (I) Mucin-2 immunofluorescence and (J) ZO-1 immunohistochemistry staining in colon tissues. Data are expressed as means ± SD (n=4-6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. **P < 0.01, *** P < 0.001, and **** P < 0.0001.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Microbiota-driven therapeutic efficacy of Hyperoside in ulcerative colitis and associated anxiety

doi: 10.3389/fcimb.2026.1734356

Figure Lengend Snippet: HYP ameliorates intestinal inflammation and restores barrier integrity in DSS-induced colitis mice. mRNA expression levels of (A) IL-6, (B) IL-1β, and (C) TNF-α in colon tissues. Levels of (E) IL-6, (F) IL-1β, and (G) TNF-α in serum. Quantitative analysis of the positive area percentage for (D) Mucin-2 and (H) ZO-1. Representative images of (I) Mucin-2 immunofluorescence and (J) ZO-1 immunohistochemistry staining in colon tissues. Data are expressed as means ± SD (n=4-6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. **P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: The following antibodies were used in this study: anti-ZO-1 antibody (AF5145, Affinity), anti-mucin-2 antibody (GB11344, Serbicebio), anti-BDNF antibody (GB11559, Serbicebio), anti-GFAP antibody (GB11096, Serbicebio), anti-Iba-1 antibody ( GB154490 , Serbicebio), NF-κB p65 (Proteintech, 10745-1-AP), Phospho-NF-κB p65 (Affinity, AF2006), AKT (Proteintech, 10176-2-AP), Phospho-AKT (Proteintech, 28731-1-AP), p38MAPK (Proteintech, 51115-1-AP), Phospho-p38MAPK (Affinity, AF4001).

Techniques: Expressing, Immunofluorescence, Immunohistochemistry, Staining